Hotspot

clpP

LSC · 73982-76081 · 2100 bp

Back to Ripogonum

Pi 0.000794

MarkerSeek score 59.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2100
variable_sites: 3
pi: 0.000794
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCGTGGAAAATTCGAATTATGGT	ACCCATTGGTACGTTTCCAT	34.8	45.0	56.9	57.1	2185-2209 bp	1.0	41.3
clpP_p2	TCGTGGAAAATTCGAATTATGGTT	ACCCATTGGTACGTTTCCAT	33.3	45.0	57.5	57.1	2185-2209 bp	1.0	41.3
clpP_p3	CGTGGAAAATTCGAATTATGGT	ACCCATTGGTACGTTTCCAT	36.4	45.0	55.4	57.1	2184-2208 bp	1.0	41.3
clpP_p4	ATCGTGGAAAATTCGAATTATGGT	ACCCATTGGTACGTTTCCAT	33.3	45.0	57.3	57.1	2186-2210 bp	1.0	41.3
clpP_p5	TCGTGGAAAATTCGAATTATGG	ACCCATTGGTACGTTTCCAT	36.4	45.0	55.2	57.1	2185-2209 bp	1.0	41.3