Hotspot

trnT_UGU-trnL_UAA

LSC · 48452-49412 · 961 bp

Back to Ripogonum

Pi 0.00513

MarkerSeek score 59.90

Primer available yes

Label trnT_UGU-trnL_UAA

Feature Metadata

label_name: trnT_UGU-trnL_UAA
feature_type: igs
region: LSC
length_bp: 961
variable_sites: 9
pi: 0.00513
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT_UGU-trnL_UAA_p1	AAAGCCAGCTATCGGAATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.1	58.2	820-1067 bp	1.0	81.7
trnT_UGU-trnL_UAA_p2	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	775-1022 bp	1.0	81.7
trnT_UGU-trnL_UAA_p3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	807-1054 bp	1.0	80.8
trnT_UGU-trnL_UAA_p4	AAAGCCAGCTATCGGAATCG	CGTCTACCGATTTCGCCATA	50.0	50.0	58.1	57.6	814-1061 bp	1.0	80.4
trnT_UGU-trnL_UAA_p5	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	769-1016 bp	1.0	80.4