Hotspot

psbM-trnD_GUC

LSC · 31225-32293 · 1069 bp

Back to Ripogonum

Pi 0.00443

MarkerSeek score 51.40

Primer available yes

Label psbM-trnD_GUC

Feature Metadata

label_name: psbM-trnD_GUC
feature_type: igs
region: LSC
length_bp: 1069
variable_sites: 11
pi: 0.00443
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_GUC_p1	AGGCGGTAGGAACTAGAATGA	CCTCGCTGGGATTGTAGTTC	47.6	55.0	58.0	58.1	1205-1216 bp	1.0	72.4
psbM-trnD_GUC_p2	AGGCGGTAGGAACTAGAATGA	AGTTCAATCGGTCAGAGCAC	47.6	50.0	58.0	57.9	1190-1201 bp	1.0	72.2
psbM-trnD_GUC_p3	AGGCGGTAGGAACTAGAATGA	TACACCTCGCTGGGATTGTA	47.6	50.0	58.0	58.1	1209-1220 bp	1.0	71.7
psbM-trnD_GUC_p4	AGGCGGTAGGAACTAGAATGA	CATACACCTCGCTGGGATTG	47.6	55.0	58.0	58.4	1211-1222 bp	1.0	69.6
psbM-trnD_GUC_p5	AGGCGGTAGGAACTAGAATGA	GTATGCCATACACCTCGCTG	47.6	55.0	58.0	58.5	1217-1228 bp	1.0	68.9