Hotspot

trnS_GCU-trnG_UCC

LSC · 8973-10044 · 1072 bp

Back to Ripogonum

Pi 0.00361
MarkerSeek score 54.40
Primer available yes
Label trnS_GCU-trnG_UCC

Feature Metadata

label_name
trnS_GCU-trnG_UCC
feature_type
igs
region
LSC
length_bp
1072
variable_sites
9
pi
0.00361
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS_GCU-trnG_UCC_p1 GATGGGGGATAACGGAGAGA ACGAATCACACTTTTACCACT 55.0 38.1 58.0 55.5 1185-1213 bp 1.0 53.8
trnS_GCU-trnG_UCC_p2 ATGGGGGATAACGGAGAGAG ACGAATCACACTTTTACCACT 55.0 38.1 58.0 55.5 1184-1212 bp 1.0 53.7
trnS_GCU-trnG_UCC_p3 ATTAGCAATCCGCCGCTTTA ACGAATCACACTTTTACCACT 45.0 38.1 58.0 55.5 1123-1151 bp 1.0 53.7
trnS_GCU-trnG_UCC_p4 CAAGTGATGGGGGATAACGG ACGAATCACACTTTTACCACT 55.0 38.1 58.0 55.5 1190-1218 bp 1.0 53.6
trnS_GCU-trnG_UCC_p5 AAGTCTCAAGTGATGGGGGA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1196-1224 bp 1.0 53.5