Hotspot

rps15-ycf1

SSC · 123223-123600 · 378 bp

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Pi 0.0534

MarkerSeek score 77.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 378
variable_sites: 35
pi: 0.0534
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGACGTTTTC	GCGTTTTCAATTGCGATGGA	50.0	45.0	58.0	58.0	1261-1299 bp	1.0	89.1
rps15-ycf1_p2	GCTGGCCCTTTTAGGAACTT	GCGTTTTCAATTGCGATGGA	50.0	45.0	58.1	58.0	1741-1785 bp	1.0	87.1
rps15-ycf1_p3	CAGCAGTCGTTGACGTTTTC	GAACCTGTCCGTTTGTCTGT	50.0	50.0	58.0	58.1	1059-1097 bp	0.75	77.0
rps15-ycf1_p4	CAGCAGTCGTTGACGTTTTC	TGAACCTGTCCGTTTGTCTG	50.0	50.0	58.0	58.1	1060-1098 bp	0.75	76.9
rps15-ycf1_p5	GCTGGCCCTTTTAGGAACTT	GAACCTGTCCGTTTGTCTGT	50.0	50.0	58.1	58.1	1539-1583 bp	0.75	75.2