Hotspot

psaJ-rpl33

LSC · 66254-66676 · 423 bp

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Pi 0.0557

MarkerSeek score 78.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 423
variable_sites: 45
pi: 0.0557
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CGAACTGAACTAAGAGCGCT	GGGGTGTTATGCCGATTCTT	50.0	50.0	58.0	57.9	1297-1316 bp	1.0	85.9
psaJ-rpl33_p2	ACCCGTGACATTTTGTACCC	GGGGTGTTATGCCGATTCTT	50.0	50.0	58.1	57.9	1073-1101 bp	1.0	84.8
psaJ-rpl33_p3	CCCGTGACATTTTGTACCCA	GGGGTGTTATGCCGATTCTT	50.0	50.0	58.1	57.9	1072-1100 bp	1.0	84.8
psaJ-rpl33_p4	ACAGGATTTGAACCCGTGAC	GGGGTGTTATGCCGATTCTT	50.0	50.0	58.1	57.9	1084-1112 bp	1.0	84.7
psaJ-rpl33_p5	CAGGATTTGAACCCGTGACA	GGGGTGTTATGCCGATTCTT	50.0	50.0	58.1	57.9	1083-1111 bp	1.0	84.7