Hotspot

ycf9-trnG

LSC · 35516-36020 · 505 bp

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Pi 0.0442

MarkerSeek score 81.70

Primer available yes

Label ycf9-trnG

Feature Metadata

label_name: ycf9-trnG
feature_type: igs
region: LSC
length_bp: 505
variable_sites: 39
pi: 0.0442
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf9-trnG_p1	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	595-658 bp	1.0	85.7
ycf9-trnG_p2	TTTGCGTCTCCTGATGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.5	57.8	603-666 bp	1.0	83.6
ycf9-trnG_p3	ATTTGCGTCTCCTGATGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.5	57.8	604-667 bp	1.0	83.5
ycf9-trnG_p4	TCTCCTGATGGTTGGTCGAG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.8	57.8	597-660 bp	1.0	81.0
ycf9-trnG_p5	CCTGATGGTTGGTCGAGTAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.9	57.8	594-657 bp	1.0	79.1