Hotspot

trnD-trnY

LSC · 29644-30059 · 416 bp

Back to Ricotia

Pi 0.0317

MarkerSeek score 72.20

Primer available yes

Label trnD-trnY

Feature Metadata

label_name: trnD-trnY
feature_type: igs
region: LSC
length_bp: 416
variable_sites: 24
pi: 0.0317
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-trnY_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	483-498 bp	1.0	82.2
trnD-trnY_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	482-497 bp	1.0	82.2
trnD-trnY_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	504-519 bp	1.0	81.1
trnD-trnY_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	503-518 bp	1.0	80.9
trnD-trnY_p5	CGGTGCTCTGACCAATTGAA	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.5	58.4	537-552 bp	1.0	80.4