Hotspot

psbI-trnS

LSC · 7647-7736 · 90 bp

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Pi 0.0574

MarkerSeek score 75.30

Primer available yes

Label psbI-trnS

Feature Metadata

label_name: psbI-trnS
feature_type: igs
region: LSC
length_bp: 90
variable_sites: 10
pi: 0.0574
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS_p1	AAATCTTGACGCATGGACGA	TAGGAGAGATGGCTGAGTGG	45.0	55.0	57.9	57.9	504-654 bp	1.0	86.5
psbI-trnS_p2	AATCTTGACGCATGGACGAA	TAGGAGAGATGGCTGAGTGG	45.0	55.0	57.9	57.9	503-653 bp	1.0	86.5
psbI-trnS_p3	AATCGTAGATGTGATGCCCG	TAGGAGAGATGGCTGAGTGG	50.0	55.0	57.8	57.9	665-815 bp	1.0	85.6
psbI-trnS_p4	GTTTGGCAAGCAGCTGTAAG	TAGGAGAGATGGCTGAGTGG	50.0	55.0	57.9	57.9	756-763 bp	0.75	72.6
psbI-trnS_p5	GTTTGGCAAGCAGCTGTAAG	TGAGTGGACTAAAGCGTTGG	50.0	50.0	57.9	57.8	743-750 bp	0.75	72.0