Hotspot

trnK-trnQ

LSC · 4317-6506 · 2190 bp

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Pi 0.0353

MarkerSeek score 73.30

Primer available yes

Label trnK-trnQ

Feature Metadata

label_name: trnK-trnQ
feature_type: igs
region: LSC
length_bp: 2190
variable_sites: 142
pi: 0.0353
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-trnQ_p1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2201-2248 bp	1.0	85.5
trnK-trnQ_p2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2200-2247 bp	1.0	85.5
trnK-trnQ_p3	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	2187-2234 bp	1.0	83.2
trnK-trnQ_p4	AGTCGCACTTAAAAGCCGAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.6	58.1	2212-2259 bp	1.0	82.3
trnK-trnQ_p5	TCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.3	58.1	2210-2257 bp	1.0	81.0