Hotspot

trnH-psbA

LSC · 77-357 · 281 bp

Back to Ricotia

Pi 0.101

MarkerSeek score 78.40

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 281
variable_sites: 48
pi: 0.101
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	CTGCCTTAATCCACTTGGCT	TGAGGCTCCATCTACAAATGG	50.0	47.6	57.9	57.4	311-334 bp	1.0	78.4
trnH-psbA_p2	ACTGCCTTAATCCACTTGGC	TGAGGCTCCATCTACAAATGG	50.0	47.6	58.2	57.4	312-335 bp	1.0	78.2
trnH-psbA_p3	AATCCACTTGGCTACATCCG	TGAGGCTCCATCTACAAATGG	50.0	47.6	57.7	57.4	304-327 bp	1.0	76.8
trnH-psbA_p4	CTGCCTTAATCCACTTGGCT	TTGAGGCTCCATCTACAAATGG	50.0	45.5	57.9	58.1	312-335 bp	1.0	74.0
trnH-psbA_p5	ACTGCCTTAATCCACTTGGC	TTGAGGCTCCATCTACAAATGG	50.0	45.5	58.2	58.1	313-336 bp	1.0	73.8