Hotspot

ycf1

SSC · 127008-132506 · 5499 bp

Pi 0.0124

MarkerSeek score 51.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5499
variable_sites: 275
pi: 0.0124
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTGTTCCCATTGCAAAATAC	GCAAGACATCAAAAGATAACTGGA	38.1	37.5	54.3	57.5	171 bp	1.0	47.5
ycf1_p2	GTTGTTCCCATTGCAAAATAC	GCAAGACATCAAAAGATAACTGG	38.1	39.1	54.3	56.2	171 bp	1.0	47.5
ycf1_p3	TTGTTCCCATTGCAAAATAC	TGCAAGACATCAAAAGATAACTGG	35.0	37.5	52.6	57.7	171 bp	1.0	47.5
ycf1_p4	GTTGTTCCCATTGCAAAATAC	TGCAAGACATCAAAAGATAACTGG	38.1	37.5	54.3	57.7	172 bp	1.0	47.4
ycf1_p5	GTTGTTCCCATTGCAAAATA	TGCAAGACATCAAAAGATAACTGG	35.0	37.5	52.6	57.7	172 bp	1.0	47.4