Hotspot

ccsA-ndhD

SSC · 118579-118805 · 227 bp

Back to Ribes

Pi 0.0315
MarkerSeek score 52.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
227
variable_sites
18
pi
0.0315
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GCATGGGGCTCATATTGGAA GGACCGCGAGAGTTATTTGT 50.0 50.0 58.0 58.0 581-597 bp 1.0 85.8
ccsA-ndhD_p2 GCATGGGGCTCATATTGGAA TTATCGATGGCTTCCCTTGC 50.0 50.0 58.0 58.0 812-828 bp 1.0 84.7
ccsA-ndhD_p3 GCATGGGGCTCATATTGGAA CTCGACGAAATGGGTGGAAT 50.0 50.0 58.0 58.0 872-888 bp 1.0 84.4
ccsA-ndhD_p4 GCATGGGGCTCATATTGGAA GCGTATGGGTTGATTCGGAT 50.0 50.0 58.0 58.1 1181-1197 bp 1.0 82.9
ccsA-ndhD_p5 GCATGGGGCTCATATTGGAA TTATTACCCCGCGCTCATTC 50.0 50.0 58.0 58.1 1151-1167 bp 1.0 82.9