Hotspot

ndhF-rpl32

SSC · 115270-116391 · 1122 bp

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Pi 0.02
MarkerSeek score 64.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1122
variable_sites
84
pi
0.02
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACGAAAGGTACGATCCATGC TTAACGCTGCCCAATATCCC 50.0 50.0 58.1 58.0 1222-1262 bp 0.933333 86.0
ndhF-rpl32_p2 CGAAAGGTACGATCCATGCA TTAACGCTGCCCAATATCCC 50.0 50.0 58.1 58.0 1221-1261 bp 0.933333 86.0
ndhF-rpl32_p3 GCCCACATACGACGAAGATT TTAACGCTGCCCAATATCCC 50.0 50.0 58.1 58.0 1303-1343 bp 0.933333 85.8
ndhF-rpl32_p4 CTGAATAGACAGATCGGCCG TTAACGCTGCCCAATATCCC 55.0 50.0 57.9 58.0 1364-1404 bp 0.933333 85.7
ndhF-rpl32_p5 TACATGAAGGGGGAATTGCG TTAACGCTGCCCAATATCCC 50.0 50.0 57.9 58.0 1925-1965 bp 0.933333 84.9