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rps8-rpl14

LSC · 83456-83603 · 148 bp

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Pi 0.0274
MarkerSeek score 57.50
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
148
variable_sites
14
pi
0.0274
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 AGCAATAGTGTCCCTACCCA AGTTTCATTAGCCCCTGAAGT 50.0 42.9 57.7 56.9 192-197 bp 0.933333 66.6
rps8-rpl14_p2 TGCTATTCGAACCGTTCCTT AGTTTCATTAGCCCCTGAAGT 45.0 42.9 57.2 56.9 252-257 bp 1.0 63.7
rps8-rpl14_p3 CAGCAATAGTGTCCCTACCC AGTTTCATTAGCCCCTGAAGT 55.0 42.9 57.4 56.9 193-198 bp 0.933333 63.7
rps8-rpl14_p4 AGCAATAGTGTCCCTACCCA GTTTCATTAGCCCCTGAAGT 50.0 45.0 57.7 55.3 191-196 bp 0.933333 62.0
rps8-rpl14_p5 ATGCTATTCGAACCGTTCCT AGTTTCATTAGCCCCTGAAGT 45.0 42.9 57.0 56.9 253-258 bp 1.0 61.9