Hotspot

accD-psaI

LSC · 61794-62472 · 679 bp

Back to Ribes

Pi 0.0186
MarkerSeek score 62.30
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
679
variable_sites
48
pi
0.0186
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CCGTTTGATCGAGTGTGCTA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 1163-1198 bp 1.0 86.2
accD-psaI_p2 TGGGATCTGTAGTAGGCGAG CCATTGCAATTGCCGGAAAT 55.0 45.0 58.0 58.0 1191-1226 bp 1.0 86.1
accD-psaI_p3 CCGTTTGATCGAGTGTGCTA TTTCCGCATGCATAAGAGCT 50.0 45.0 58.0 58.0 1349-1382 bp 0.866667 79.7
accD-psaI_p4 TGGGATCTGTAGTAGGCGAG TTTCCGCATGCATAAGAGCT 55.0 45.0 58.0 58.0 1377-1410 bp 0.866667 79.5
accD-psaI_p5 CCGTTTGATCGAGTGTGCTA TCCGCGGATCCTGATCTATT 50.0 50.0 58.0 58.1 1405-1438 bp 0.866667 79.5