Hotspot

ndhC-trnV-UAC

LSC · 53647-54537 · 891 bp

Back to Ribes

Pi 0.0139
MarkerSeek score 72.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
891
variable_sites
55
pi
0.0139
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CGCCGGAAATTAGAAATGCC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 833-1014 bp 1.0 86.0
ndhC-trnV-UAC_p2 GCCCTTTGTTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 857-1038 bp 1.0 84.9
ndhC-trnV-UAC_p3 TCTCCGGCCCTTTGTTAATC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.6 57.8 863-1044 bp 1.0 83.5
ndhC-trnV-UAC_p4 CGCCGGAAATTAGAAATGCC GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 832-1013 bp 1.0 83.3
ndhC-trnV-UAC_p5 GCCCTTTGTTAATCGGAGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.2 57.5 856-1037 bp 1.0 82.1