Hotspot

ycf3-trnS-GGA

LSC · 46977-47811 · 835 bp

Back to Ribes

Pi 0.0187
MarkerSeek score 66.90
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
835
variable_sites
55
pi
0.0187
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 47.6 50.0 58.0 58.1 831-931 bp 1.0 79.7
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 852-952 bp 1.0 79.2
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 47.6 45.0 58.0 57.8 802-902 bp 1.0 79.1
ycf3-trnS-GGA_p4 CATTTATCCGCGATCTAGGCA ACCCTCGGTAAACAAAAGCC 47.6 50.0 58.0 58.4 832-932 bp 1.0 77.4
ycf3-trnS-GGA_p5 TTTATCCGCGATCTAGGCAT CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.6 58.1 829-929 bp 1.0 76.7