Hotspot

trnT-GGU-psbD

LSC · 33918-35173 · 1256 bp

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Pi 0.0172
MarkerSeek score 65.30
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1256
variable_sites
96
pi
0.0172
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 50.0 45.0 57.9 58.2 1413-1463 bp 1.0 85.8
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA GACCGGACCAACCTACAAAA 50.0 50.0 57.9 57.7 1427-1477 bp 1.0 85.7
trnT-GGU-psbD_p3 GCCCTTTTAACTCAGCGGTA TAGACCGGACCAACCTACAA 50.0 50.0 57.9 57.7 1429-1479 bp 1.0 85.3
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA GAGCAATAGACCGGACCAAC 50.0 55.0 57.9 58.3 1435-1485 bp 1.0 84.9
trnT-GGU-psbD_p5 GCCCTTTTAACTCAGCGGTA CAAAAACGAAACGGTCCCTC 50.0 50.0 57.9 57.6 1412-1462 bp 1.0 84.6