Hotspot

atpH-atpI

LSC · 14925-16035 · 1111 bp

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Pi 0.0161
MarkerSeek score 64.70
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1111
variable_sites
69
pi
0.0161
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TTTGCAACTTTAGCTGCAGC 45.0 45.0 58.0 57.9 1175-1206 bp 1.0 87.4
atpH-atpI_p2 AATAACGGAAGCGGCAGAAA TTTGCAACTTTAGCTGCAGC 45.0 45.0 58.2 57.9 1176-1207 bp 1.0 86.2
atpH-atpI_p3 GCCAATCCAGCAGCAATAAC TTTGCAACTTTAGCTGCAGC 50.0 45.0 57.8 57.9 1190-1221 bp 1.0 85.9
atpH-atpI_p4 TCCAGCAGCAATAACGGAAG TTTGCAACTTTAGCTGCAGC 50.0 45.0 58.3 57.9 1185-1216 bp 1.0 85.5
atpH-atpI_p5 ATCCAGCAGCAATAACGGAA TTTGCAACTTTAGCTGCAGC 45.0 45.0 57.6 57.9 1186-1217 bp 1.0 84.3