Hotspot

trnH-GUG-psbA

LSC · 94-442 · 349 bp

Back to Ribes

Pi 0.0233
MarkerSeek score 63.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
349
variable_sites
37
pi
0.0233
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GAAGCTCCATCTACAAATGG 55.0 45.0 58.1 53.8 395-419 bp 0.866667 49.2
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GAAGCTCCATCTACAAATGG 50.0 45.0 57.9 53.8 409-433 bp 0.866667 48.9
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC GAAGCTCCATCTACAAATGG 50.0 45.0 57.9 53.8 408-432 bp 0.866667 48.9
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GAAGCTCCATCTACAAATGG 50.0 45.0 57.9 53.8 410-434 bp 0.866667 48.9
trnH-GUG-psbA_p5 CGCATGGTGGATTCACAATC GAAGCTCCATCTACAAATGG 50.0 45.0 57.5 53.8 424-448 bp 0.866667 45.8