Hotspot

ycf1

SSC · 124441-129921 · 5481 bp

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Pi 0.00529

MarkerSeek score 41.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5481
variable_sites: 29
pi: 0.00529
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGCCTGTCTCGTTAAATCGA	GCAGAATACCGTCACCCATT	45.0	50.0	56.7	58.0	258 bp	1.0	70.7
ycf1_p2	AGCCTGTCTCGTTAAATCGAA	GCAGAATACCGTCACCCATT	42.9	50.0	57.4	58.0	258 bp	1.0	68.5
ycf1_p3	GCCTGTCTCGTTAAATCGAAG	GCAGAATACCGTCACCCATT	47.6	50.0	57.1	58.0	257 bp	1.0	66.0
ycf1_p4	GCCTGTCTCGTTAAATCGAA	GCAGAATACCGTCACCCATT	45.0	50.0	55.9	58.0	257 bp	1.0	64.4
ycf1_p5	CCTGTCTCGTTAAATCGAAGGT	GCAGAATACCGTCACCCATT	45.5	50.0	58.2	58.0	256 bp	1.0	64.0