Hotspot

psbB

LSC · 74099-75625 · 1527 bp

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Pi 0.00984

MarkerSeek score 57.60

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 15
pi: 0.00984
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	CCATTGAACAAGGGAGGTCC	GCACTCTGGTACTCTGATGTC	55.0	52.4	58.2	57.8	1701-1703 bp	1.0	70.3
psbB_p2	CCATTGAACAAGGGAGGTCC	AGCGTTGATTCAAATGCACTC	55.0	42.9	58.2	57.8	1716-1718 bp	1.0	69.8
psbB_p3	CCATTGAACAAGGGAGGTCC	GCGTTGATTCAAATGCACTCT	55.0	42.9	58.2	57.8	1715-1717 bp	1.0	69.8
psbB_p4	CCATTGAACAAGGGAGGTCC	TGCACTCTGGTACTCTGATG	55.0	50.0	58.2	56.7	1702-1704 bp	1.0	68.8
psbB_p5	CCATTGAACAAGGGAGGTCC	GCACTCTGGTACTCTGATGT	55.0	50.0	58.2	56.7	1701-1703 bp	1.0	68.8