Hotspot

ccsA

SSC · 115083-116045 · 963 bp

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Pi 0.00208

MarkerSeek score 46.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 963
variable_sites: 2
pi: 0.00208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TAGACACGCTGCTCTTAGGA	TCAGTAGCTTTTCGATGGCC	50.0	50.0	57.9	58.0	1724-1725 bp	1.0	79.6
ccsA_p2	TAGACACGCTGCTCTTAGGA	CAGTAGCTTTTCGATGGCCT	50.0	50.0	57.9	58.0	1723-1724 bp	1.0	79.6
ccsA_p3	AAATTGGTAGACACGCTGCT	TCAGTAGCTTTTCGATGGCC	45.0	50.0	57.8	58.0	1731-1732 bp	1.0	79.2
ccsA_p4	AAATTGGTAGACACGCTGCT	CAGTAGCTTTTCGATGGCCT	45.0	50.0	57.8	58.0	1730-1731 bp	1.0	79.1
ccsA_p5	TAGACACGCTGCTCTTAGGA	TTCTCGCCTGTTCACACAAA	50.0	45.0	57.9	58.0	1199 bp	1.0	79.0