Hotspot

rpl16-rps3

LSC · 83212-84259 · 1048 bp

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Pi 0.00382

MarkerSeek score 60.90

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1048
variable_sites: 4
pi: 0.00382
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GTTCAAGTGCCTGAAGAGCA	GGTTCCCCTCCAAACCATTC	50.0	55.0	58.4	58.4	1281 bp	1.0	73.5
rpl16-rps3_p2	GGGTTCAAGTGCCTGAAGAG	GGTTCCCCTCCAAACCATTC	55.0	55.0	58.5	58.4	1283 bp	1.0	72.9
rpl16-rps3_p3	TTCAAGTGCCTGAAGAGCAT	GGTTCCCCTCCAAACCATTC	45.0	55.0	57.4	58.4	1280 bp	1.0	72.1
rpl16-rps3_p4	GTTCAAGTGCCTGAAGAGCA	CCTCCAAACCATTCGAGCTA	50.0	50.0	58.4	57.3	1275 bp	1.0	71.5
rpl16-rps3_p5	GGGTTCAAGTGCCTGAAGAG	CCTCCAAACCATTCGAGCTA	55.0	50.0	58.5	57.3	1277 bp	1.0	70.9