Hotspot

infA-rps8

LSC · 81618-81725 · 108 bp

Back to Rhynchotechum

Pi 0.0185

MarkerSeek score 63.10

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 108
variable_sites: 2
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	CCGTTGGGAAGTGATTCAGT	GAAAGAATCGGCGGAGAGTT	50.0	50.0	57.8	58.0	203 bp	1.0	79.2
infA-rps8_p2	CCGTTGGGAAGTGATTCAGT	CGGGAAGCTCGACTAGAAAG	50.0	55.0	57.8	57.8	218 bp	1.0	77.7
infA-rps8_p3	CCGTTGGGAAGTGATTCAGT	AAAGAATCGGCGGAGAGTTG	50.0	50.0	57.8	58.3	202 bp	1.0	77.1
infA-rps8_p4	ATGCCGTTGGGAAGTGATTC	GAAAGAATCGGCGGAGAGTT	50.0	50.0	58.5	58.0	206 bp	1.0	76.4
infA-rps8_p5	CCGTTGGGAAGTGATTCAGT	GCGGAGAGTTGTTGTGTTCT	50.0	50.0	57.8	58.4	193 bp	1.0	76.0