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petB-petD

LSC · 77924-78857 · 934 bp

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Pi 0.00754

MarkerSeek score 49.40

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 934
variable_sites: 7
pi: 0.00754
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	TATCGTCCGACCGTTACTGA	CGACTGCAGTACCGATCAAA	50.0	50.0	58.0	58.0	1866-1871 bp	1.0	80.4
petB-petD_p2	ACTATCGTCCGACCGTTACT	CGACTGCAGTACCGATCAAA	50.0	50.0	58.0	58.0	1868-1873 bp	1.0	80.4
petB-petD_p3	GCCGCTTCTTACTGCTGTAT	CGACTGCAGTACCGATCAAA	50.0	50.0	58.1	58.0	1465-1470 bp	1.0	80.3
petB-petD_p4	GTCTCGAGATTCAGGCGATC	CGACTGCAGTACCGATCAAA	55.0	50.0	58.0	58.0	2006-2011 bp	1.0	80.2
petB-petD_p5	TATCGTCCGACCGTTACTGA	TGCAAATGGATCTGCTGGTT	50.0	45.0	58.0	58.1	1658-1663 bp	1.0	80.0