Hotspot

petA-psbJ

LSC · 64761-65840 · 1080 bp

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Pi 0.00463

MarkerSeek score 61.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1080
variable_sites: 5
pi: 0.00463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1137-1138 bp	1.0	75.0
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	1139-1140 bp	1.0	75.0
petA-psbJ_p3	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	1136-1137 bp	1.0	74.5
petA-psbJ_p4	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.2	57.4	1138-1139 bp	1.0	74.5
petA-psbJ_p5	CGCGTCCAGGGTCTTTTATT	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.3	57.5	1215-1216 bp	1.0	74.3