Hotspot

accD-psaI

LSC · 60217-60932 · 716 bp

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Pi 0.00559

MarkerSeek score 62.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 4
pi: 0.00559
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TAAGCTCCACGCCTTTTTCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.5	58.0	811 bp	1.0	76.4
accD-psaI_p2	TAAGCTCCACGCCTTTTTCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.5	57.9	817 bp	1.0	75.8
accD-psaI_p3	GGTTCACAAGCGGCTGAATA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.6	58.0	903 bp	1.0	75.7
accD-psaI_p4	GGTTCACAAGCGGCTGAATA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.6	57.9	909 bp	1.0	75.1
accD-psaI_p5	TAAGCTCCACGCCTTTTTCC	GCCGGAAATACTAGGCCTAC	50.0	55.0	58.5	57.5	800 bp	1.0	73.0