Hotspot

petN-psbM

LSC · 29601-30620 · 1020 bp

Back to Rhynchotechum

Pi 0.00296

MarkerSeek score 60.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1020
variable_sites: 3
pi: 0.00296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACCGCCT	50.0	47.6	58.0	58.0	1112-1116 bp	1.0	72.3
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCCT	50.0	47.6	58.0	58.0	1110-1114 bp	1.0	72.3
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	TCTAGTTCCTACCGCCTTTCT	50.0	47.6	58.0	57.9	1108-1112 bp	1.0	71.8
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	TCTAGTTCCTACCGCCTTTCT	50.0	47.6	58.0	57.9	1106-1110 bp	1.0	71.8
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	TCATTCTAGTTCCTACCGCCT	55.0	47.6	57.9	58.0	1109-1113 bp	1.0	71.7