Hotspot

matK-trnK-UUU

LSC · 3530-4236 · 707 bp

Back to Rhynchotechum

Pi 0.00566

MarkerSeek score 62.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 4
pi: 0.00566
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCATGATCGTGAGCAAATGC	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.9	58.2	838-839 bp	1.0	78.1
matK-trnK-UUU_p2	CCATGATCGTGAGCAAATGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.9	58.2	837-838 bp	1.0	78.1
matK-trnK-UUU_p3	CCATGATCGTGAGCAAATGC	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.9	57.5	851-852 bp	1.0	75.8
matK-trnK-UUU_p4	CCATGATCGTGAGCAAATGC	TTCGGGTTGCTAACTCAACG	50.0	50.0	57.9	58.5	854-855 bp	1.0	75.5
matK-trnK-UUU_p5	TGAAACCATGATCGTGAGCA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.5	58.2	842-843 bp	1.0	75.3