Hotspot

ccsA-ndhD

SSC · 120139-120489 · 351 bp

Pi 0.0375

MarkerSeek score 72.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 351
variable_sites: 29
pi: 0.0375
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGCGCTATTTTTACCCAGGG	TTATCGATGGCTTCCCTTGC	50.0	50.0	57.9	58.0	1318-1402 bp	1.0	85.3
ccsA-ndhD_p2	AGCGCTATTTTTACCCAGGG	CGCTGCACTCTTTTTCTTGG	50.0	50.0	57.9	58.0	1433-1517 bp	1.0	84.9
ccsA-ndhD_p3	AGCGCTATTTTTACCCAGGG	TATCTTGACGAAATGGGCGG	50.0	50.0	57.9	58.1	1381-1465 bp	1.0	84.9
ccsA-ndhD_p4	AGCGCTATTTTTACCCAGGG	ATGGGTTTATTAGCGCTGCA	50.0	45.0	57.9	57.9	1446-1530 bp	1.0	84.8
ccsA-ndhD_p5	AGCGCTATTTTTACCCAGGG	TGACGAAATGGGCGGATTAG	50.0	50.0	57.9	58.1	1429-1442 bp	0.25	49.9