Hotspot

rpl32-trnL-UAG

SSC · 117865-118986 · 1122 bp

Back to Rhus

Pi 0.0346
MarkerSeek score 77.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1122
variable_sites
123
pi
0.0346
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCATGCCGAAACTAGACAGA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 1578-2126 bp 1.0 91.4
rpl32-trnL-UAG_p2 GCATGCCGAAACTAGACAGA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 1579-2127 bp 1.0 91.4
rpl32-trnL-UAG_p3 AGCATGCCGAAACTAGACAG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 1579-2127 bp 1.0 91.3
rpl32-trnL-UAG_p4 AGCATGCCGAAACTAGACAG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 1580-2128 bp 1.0 91.3
rpl32-trnL-UAG_p5 TAGCGAAATCCCTTTCCACG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 895-1225 bp 0.625 72.1