Hotspot

rpl22-rps19

LSC · 87694-87738 · 45 bp

Back to Rhus

Pi 0.0833
MarkerSeek score 62.30
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
45
variable_sites
15
pi
0.0833
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TAAGAACGCCCACGAATCTG GCCGCCGTAGTAAATAGGAG 50.0 55.0 58.0 57.9 245-533 bp 1.0 88.3
rpl22-rps19_p2 CTCATAAGAACGCCCACGAA GGAAGGGCTCCAATTGGTAG 50.0 55.0 58.0 57.9 355-643 bp 1.0 87.4
rpl22-rps19_p3 TAAGAACGCCCACGAATCTG GGAAGGGCTCCAATTGGTAG 50.0 55.0 58.0 57.9 351-639 bp 1.0 87.4
rpl22-rps19_p4 CTCATAAGAACGCCCACGAA CCGGATCGAAATGTTGGCTA 50.0 50.0 58.0 58.1 441-729 bp 1.0 86.9
rpl22-rps19_p5 TAAGAACGCCCACGAATCTG CCGGATCGAAATGTTGGCTA 50.0 50.0 58.0 58.1 437-725 bp 1.0 86.9