Hotspot

ycf4-cemA

LSC · 63674-64645 · 972 bp

Back to Rhus

Pi 0.0258
MarkerSeek score 64.00
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
972
variable_sites
59
pi
0.0258
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGACTCCGCGAGAAATTGAG GGATATGATCCTCGTCGTGC 50.0 55.0 58.0 58.0 1257-1368 bp 1.0 87.8
ycf4-cemA_p2 TGAGAATTTGACTCCGCGAG GGATATGATCCTCGTCGTGC 50.0 55.0 58.0 58.0 1265-1376 bp 1.0 87.8
ycf4-cemA_p3 TTAGCCTATTTCTTGCGCGT GGATATGATCCTCGTCGTGC 45.0 55.0 58.0 58.0 1222-1333 bp 1.0 87.8
ycf4-cemA_p4 TGACTCCGCGAGAAATTGAG AGATGCCTTTCCGGGAATTC 50.0 50.0 58.0 57.9 1186-1297 bp 1.0 87.6
ycf4-cemA_p5 TGAGAATTTGACTCCGCGAG AGATGCCTTTCCGGGAATTC 50.0 50.0 58.0 57.9 1194-1305 bp 1.0 87.6