Hotspot

accD-psaI

LSC · 61823-62540 · 718 bp

Back to Rhus

Pi 0.013
MarkerSeek score 61.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
718
variable_sites
27
pi
0.013
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CCTATATTGCCTTTGCGGGT CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 975-1011 bp 1.0 84.8
accD-psaI_p2 GCCGAACCGAATGCCTATAT CCATTGCAATTGCCGGAAAT 50.0 45.0 57.9 58.0 988-1024 bp 1.0 84.1
accD-psaI_p3 ACCCGTTTGATCGAGTATGC CCATTGCAATTGCCGGAAAT 50.0 45.0 58.1 58.0 1219-1255 bp 1.0 83.4
accD-psaI_p4 TATTCTGGTGTGTGCTTCCG CCATTGCAATTGCCGGAAAT 50.0 45.0 57.9 58.0 1178-1214 bp 1.0 83.3
accD-psaI_p5 GACGGGATTAACAGAGGCTG CCATTGCAATTGCCGGAAAT 55.0 45.0 58.1 58.0 1343-1379 bp 1.0 83.1