Hotspot

ndhC-trnV-UAC

LSC · 53924-54432 · 509 bp

Back to Rhus

Pi 0.0109
MarkerSeek score 65.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
509
variable_sites
16
pi
0.0109
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TAATCGGGGCTAAAACTGCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 553-686 bp 1.0 81.7
ndhC-trnV-UAC_p2 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 560-693 bp 1.0 81.7
ndhC-trnV-UAC_p3 TAATCGGGGCTAAAACTGCG GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.3 57.5 552-685 bp 1.0 79.0
ndhC-trnV-UAC_p4 CCTTTGCTAATCGGGGCTAA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 559-692 bp 1.0 78.9
ndhC-trnV-UAC_p5 CTCTGGCCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.7 57.8 567-700 bp 1.0 78.8