Hotspot

trnR-UCU-atpA

LSC · 11580-11763 · 184 bp

Back to Rhus

Pi 0.035
MarkerSeek score 67.00
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
184
variable_sites
16
pi
0.035
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 ATGAAAGGCGTCCATTGTCT TGGAACGCTTTCTACTTCAGG 45.0 47.6 57.5 58.0 295-313 bp 1.0 73.5
trnR-UCU-atpA_p2 GGAATGAAAGGCGTCCATTG TGGAACGCTTTCTACTTCAGG 50.0 47.6 57.4 58.0 298-316 bp 1.0 72.9
trnR-UCU-atpA_p3 ATGAAAGGCGTCCATTGTCT GGAACGCTTTCTACTTCAGGA 45.0 47.6 57.5 57.7 294-312 bp 1.0 71.4
trnR-UCU-atpA_p4 GGAATGAAAGGCGTCCATTG GGAACGCTTTCTACTTCAGGA 50.0 47.6 57.4 57.7 297-315 bp 1.0 70.8
trnR-UCU-atpA_p5 ATGAAAGGCGTCCATTGTCT TCAGGAACATATGGAACGCT 45.0 45.0 57.5 56.6 306-324 bp 1.0 70.7