Hotspot

trnP-UGG-psaB

Genome · 120169-120421 · 253 bp

Back to Rhopalodia

Pi 0.0935

MarkerSeek score 78.10

Primer available yes

Label trnP-UGG-psaB

Feature Metadata

label_name: trnP-UGG-psaB
feature_type: igs
region: Genome
length_bp: 253
variable_sites: 35
pi: 0.0935
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaB_p1	CCAAGAACACTTCCCACCAA	TTCGTTGGCGTGATAAACCA	50.0	45.0	57.9	58.1	865-889 bp	1.0	85.0
trnP-UGG-psaB_p2	CCAAGAACACTTCCCACCAA	GTTCGTTGGCGTGATAAACC	50.0	50.0	57.9	57.8	866-890 bp	1.0	84.0
trnP-UGG-psaB_p3	CCAAGAACACTTCCCACCAA	CGCAGCCTTTGTTATTGCTT	50.0	45.0	57.9	57.6	774-798 bp	1.0	83.7
trnP-UGG-psaB_p4	CCAAGAACACTTCCCACCAA	GCACATGAGCGTACTCCATT	50.0	50.0	57.9	58.3	896-920 bp	1.0	83.0
trnP-UGG-psaB_p5	CCAAGAACACTTCCCACCAA	ATCTCATGGCGTGGTTACTG	50.0	50.0	57.9	57.7	944-968 bp	1.0	82.9