Hotspot

ycf35-rrn5

Genome · 112968-113742 · 775 bp

Back to Rhopalodia

Pi 0.102

MarkerSeek score 83.70

Primer available yes

Label ycf35-rrn5

Feature Metadata

label_name: ycf35-rrn5
feature_type: igs
region: Genome
length_bp: 775
variable_sites: 105
pi: 0.102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf35-rrn5_p1	ACCTCGCCAGCATATTGTTT	CGACGACAATACTTGGAGGG	45.0	55.0	57.9	58.1	1044-1105 bp	0.666667	71.8
ycf35-rrn5_p2	ACCTCGCCAGCATATTGTTT	TTCGGTCCATATCCGGTGTA	45.0	50.0	57.9	57.9	1514-1575 bp	0.666667	70.6
ycf35-rrn5_p3	ACCTCGCCAGCATATTGTTT	ATCTCCCCCAAGAGTTCACA	45.0	50.0	57.9	58.0	1659-1720 bp	0.666667	70.3
ycf35-rrn5_p4	ACCTCGCCAGCATATTGTTT	TCTCCCCCAAGAGTTCACAT	45.0	50.0	57.9	58.0	1658-1719 bp	0.666667	70.3
ycf35-rrn5_p5	ACCTCGCCAGCATATTGTTT	TAGTGATCTGACGGTGCTGA	45.0	50.0	57.9	58.2	1731-1792 bp	0.666667	69.2