Hotspot

rpl32-ycf35

Genome · 112056-112595 · 540 bp

Back to Rhopalodia

Pi 0.103

MarkerSeek score 80.70

Primer available yes

Label rpl32-ycf35

Feature Metadata

label_name: rpl32-ycf35
feature_type: igs
region: Genome
length_bp: 540
variable_sites: 71
pi: 0.103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ycf35_p1	GGTGGAGAGACTCGAACTCT	GTCGTCACAAGGGCTTAGAG	55.0	55.0	58.2	58.0	1509-1624 bp	0.666667	69.7
rpl32-ycf35_p2	GGACCTAAATCTAGCGCGTC	GTCGTCACAAGGGCTTAGAG	55.0	55.0	58.2	58.0	1472-1587 bp	0.666667	69.3
rpl32-ycf35_p3	TGGCTAGCTCCTACTGTCAA	GTCGTCACAAGGGCTTAGAG	50.0	55.0	57.8	58.0	1847-1962 bp	0.666667	68.7
rpl32-ycf35_p4	TTGGCTAGCTCCTACTGTCA	GTCGTCACAAGGGCTTAGAG	50.0	55.0	57.8	58.0	1848-1963 bp	0.666667	68.7
rpl32-ycf35_p5	GGTGGAGAGACTCGAACTCT	AAACAATATGCTGGCGAGGT	55.0	45.0	58.2	57.9	981-1114 bp	0.666667	68.7