Hotspot

rpl35-psaE

Genome · 60708-61627 · 920 bp

Back to Rhopalodia

Pi 0.156

MarkerSeek score 82.10

Primer available yes

Label rpl35-psaE

Feature Metadata

label_name: rpl35-psaE
feature_type: igs
region: Genome
length_bp: 920
variable_sites: 181
pi: 0.156
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl35-psaE_p1	TTTGAAGCTGCCTTTCGAGT	GGGACTTGAAGCCTCAACAA	45.0	50.0	58.0	58.0	1288-1570 bp	1.0	91.6
rpl35-psaE_p2	AAGCATGACGACGCAAAAAG	GGGACTTGAAGCCTCAACAA	45.0	50.0	58.0	58.0	1335-1617 bp	1.0	91.6
rpl35-psaE_p3	TTTGAAGCTGCCTTTCGAGT	CCCGAATTAATTGAGGGCGA	45.0	50.0	58.0	58.0	1319-1576 bp	0.666667	72.5
rpl35-psaE_p4	AAGCATGACGACGCAAAAAG	CCCGAATTAATTGAGGGCGA	45.0	50.0	58.0	58.0	1366-1623 bp	0.666667	72.4
rpl35-psaE_p5	TCCACTAGCCAGCGATAAGA	GGGACTTGAAGCCTCAACAA	50.0	50.0	57.9	58.0	1542-1799 bp	0.666667	72.4