Hotspot

atpA-ycf45

Genome · 57778-58706 · 929 bp

Back to Rhopalodia

Pi 0.16

MarkerSeek score 84.50

Primer available yes

Label atpA-ycf45

Feature Metadata

label_name: atpA-ycf45
feature_type: igs
region: Genome
length_bp: 929
variable_sites: 214
pi: 0.16
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-ycf45_p1	CTCCAGGACGTGAAGCTTAC	GATGAAGCAAAACGACGTGG	55.0	50.0	58.0	58.0	2194-2198 bp	1.0	89.6
atpA-ycf45_p2	CTCCAGGACGTGAAGCTTAC	CTTGAAGTTTTAGCCGCACG	55.0	50.0	58.0	58.0	2338-2342 bp	1.0	89.5
atpA-ycf45_p3	CTCCAGGACGTGAAGCTTAC	AACGAAAAGCATACCCAGCA	55.0	45.0	58.0	58.1	2154-2158 bp	1.0	89.3
atpA-ycf45_p4	GTATGACGGCCCTTCCAATT	GATGAAGCAAAACGACGTGG	50.0	50.0	57.9	58.0	2098-2100 bp	0.666667	68.8
atpA-ycf45_p5	CTCCAGGACGTGAAGCTTAC	GAAGTTTTAGCCGCACGAAC	55.0	50.0	58.0	58.1	2335 bp	0.333333	52.9