Hotspot

rps2-rbcS

Genome · 48380-48745 · 366 bp

Back to Rhopalodia

Pi 0.147

MarkerSeek score 73.90

Primer available yes

Label rps2-rbcS

Feature Metadata

label_name: rps2-rbcS
feature_type: igs
region: Genome
length_bp: 366
variable_sites: 79
pi: 0.147
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rbcS_p1	GCAACATTGCGTCGAAAAGA	ACACAAGGTTGCTTCTCGTT	45.0	45.0	58.0	58.0	1059-1105 bp	1.0	86.6
rps2-rbcS_p2	GCAACATTGCGTCGAAAAGA	ACAACACCTACAGCAAACGT	45.0	45.0	58.0	58.0	1121-1167 bp	1.0	86.3
rps2-rbcS_p3	GCAACATTGCGTCGAAAAGA	TTAGCTCGTAACGAAGGTGC	45.0	50.0	58.0	58.0	1277-1323 bp	1.0	85.8
rps2-rbcS_p4	AGAAGACGCCGCTAGAAATG	TTAGCTCGTAACGAAGGTGC	50.0	50.0	58.1	58.0	1506 bp	0.333333	52.7
rps2-rbcS_p5	AGAAGACGCCGCTAGAAATG	ACAACACCTACAGCAAACGT	50.0	45.0	58.1	58.0	1350 bp	0.333333	52.5