Hotspot

trnM-CAU-trnS-GCU

Genome · 17237-17302 · 66 bp

Back to Rhopalodia

Pi 0.157

MarkerSeek score 78.60

Primer available yes

Label trnM-CAU-trnS-GCU

Feature Metadata

label_name: trnM-CAU-trnS-GCU
feature_type: igs
region: Genome
length_bp: 66
variable_sites: 15
pi: 0.157
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-trnS-GCU_p1	AGTGGTTAGAGCAGGGGATT	CTTTCGACCACTCAGCCATT	50.0	50.0	58.0	58.2	151 bp	1.0	86.2
trnM-CAU-trnS-GCU_p2	AGTGGTTAGAGCAGGGGATT	TTCGACCACTCAGCCATTTC	50.0	50.0	58.0	58.2	149 bp	1.0	86.2
trnM-CAU-trnS-GCU_p3	TGGTTAGAGCAGGGGATTCA	CTTTCGACCACTCAGCCATT	50.0	50.0	58.0	58.2	149 bp	1.0	86.2
trnM-CAU-trnS-GCU_p4	TGGTTAGAGCAGGGGATTCA	TTCGACCACTCAGCCATTTC	50.0	50.0	58.0	58.2	147 bp	1.0	86.2
trnM-CAU-trnS-GCU_p5	AGTGGTTAGAGCAGGGGATT	GACCACTCAGCCATTTCTCC	50.0	55.0	58.0	58.3	146 bp	1.0	85.7