Hotspot

trnT-UGU-trnR-UCU

Genome · 16255-16402 · 148 bp

Back to Rhopalodia

Pi 0.117

MarkerSeek score 83.60

Primer available yes

Label trnT-UGU-trnR-UCU

Feature Metadata

label_name: trnT-UGU-trnR-UCU
feature_type: igs
region: Genome
length_bp: 148
variable_sites: 24
pi: 0.117
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnR-UCU_p1	GGTTCAAGTCCCTTCACCAG	TAGGGCCTATCGTCTAACGG	55.0	55.0	58.1	58.1	239-245 bp	1.0	89.3
trnT-UGU-trnR-UCU_p2	GTAGGTTGGGGGTTCAAGTC	TAGGGCCTATCGTCTAACGG	55.0	55.0	57.8	58.1	249-255 bp	1.0	88.1
trnT-UGU-trnR-UCU_p3	GGTTCAAGTCCCTTCACCAG	TTCGATTCCTACTGGGCCTA	55.0	50.0	58.1	57.5	184-190 bp	1.0	87.3
trnT-UGU-trnR-UCU_p4	GTAGGTTGGGGGTTCAAGTC	TTCGATTCCTACTGGGCCTA	55.0	50.0	57.8	57.5	194-200 bp	1.0	86.4
trnT-UGU-trnR-UCU_p5	CTCAATGGTAGAGCAACGGA	TAGGGCCTATCGTCTAACGG	50.0	55.0	57.3	58.1	279-285 bp	1.0	83.2