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psb30-trnK-UUU

Genome · 12201-12572 · 372 bp

Back to Rhopalodia

Pi 0.115

MarkerSeek score 83.20

Primer available yes

Label psb30-trnK-UUU

Feature Metadata

label_name: psb30-trnK-UUU
feature_type: igs
region: Genome
length_bp: 372
variable_sites: 61
pi: 0.115
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psb30-trnK-UUU_p1	ACCTGTAGCGATAAGTTGTCC	GGGACTCGAACCCAGAACTA	47.6	55.0	57.5	58.4	453-488 bp	1.0	76.3
psb30-trnK-UUU_p2	AGTTGTCCTAGAACTTGCCA	GGGACTCGAACCCAGAACTA	45.0	55.0	56.4	58.4	440-475 bp	1.0	75.5
psb30-trnK-UUU_p3	ACCTGTAGCGATAAGTTGTCC	CGAACCCAGAACTATTCGGT	47.6	50.0	57.5	57.3	447-482 bp	1.0	74.5
psb30-trnK-UUU_p4	CCTGTAGCGATAAGTTGTCCT	GGGACTCGAACCCAGAACTA	47.6	55.0	57.2	58.4	452-487 bp	1.0	74.0
psb30-trnK-UUU_p5	AGTTGTCCTAGAACTTGCCA	CGAACCCAGAACTATTCGGT	45.0	50.0	56.4	57.3	434-469 bp	1.0	73.7