Hotspot

cbbx-psaL

Genome · 7868-7960 · 93 bp

Back to Rhopalodia

Pi 0.136

MarkerSeek score 78.10

Primer available yes

Label cbbx-psaL

Feature Metadata

label_name: cbbx-psaL
feature_type: igs
region: Genome
length_bp: 93
variable_sites: 17
pi: 0.136
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cbbx-psaL_p1	CTCCGCCCATAGCTTTCTTT	AGGTCATTTAGCAACGCCAA	50.0	45.0	58.0	58.1	879-884 bp	1.0	84.1
cbbx-psaL_p2	CTCCGCCCATAGCTTTCTTT	TAGCAACGCCAATCACATCA	50.0	45.0	58.0	57.9	871-876 bp	1.0	84.0
cbbx-psaL_p3	CTCCGCCCATAGCTTTCTTT	GCAACGCCAATCACATCATC	50.0	50.0	58.0	58.2	869-874 bp	1.0	83.5
cbbx-psaL_p4	CTCCGCCCATAGCTTTCTTT	AGTGCAAAGCAGAACCCTAC	50.0	50.0	58.0	58.1	1155-1156 bp	0.666667	66.7
cbbx-psaL_p5	CTCCGCCCATAGCTTTCTTT	GTGCAAAGCAGAACCCTACT	50.0	50.0	58.0	58.1	1154-1155 bp	0.666667	66.7