Hotspot

rpoC1

LSC · 21430-24248 · 2819 bp

Back to Rhopaloblaste

Pi 0

MarkerSeek score 56.00

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2819
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TGAAACTGGAGAGACCCGAA	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.3	58.0	2976-2977 bp	1.0	77.4
rpoC1_p2	TGAAACTGGAGAGACCCGAA	TTCGATTGCTCGTTCGAGAA	50.0	45.0	58.3	57.7	2981-2982 bp	1.0	75.0
rpoC1_p3	TGAAACTGGAGAGACCCGAA	TTTCGATTGCTCGTTCGAGA	50.0	45.0	58.3	57.7	2982-2983 bp	1.0	75.0
rpoC1_p4	TGAAACTGGAGAGACCCGAA	CGATTGCTCGTTCGAGAACT	50.0	50.0	58.3	58.4	2979-2980 bp	1.0	74.7
rpoC1_p5	TGAAACTGGAGAGACCCGAA	TCGATTGCTCGTTCGAGAAC	50.0	50.0	58.3	58.4	2980-2981 bp	1.0	74.6