Hotspot

psbA

LSC · 241-1344 · 1104 bp

Back to Rhopaloblaste

Pi 0.00908

MarkerSeek score 51.50

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1104
variable_sites: 10
pi: 0.00908
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TCAACGATTCATATACACTAAGACA	TGAGATTGGTTGACATTGGCA	32.0	42.9	55.9	58.1	1223 bp	1.0	40.7
psbA_p2	CAACGATTCATATACACTAAGACA	TGAGATTGGTTGACATTGGCA	33.3	42.9	54.6	58.1	1222 bp	1.0	40.7
psbA_p3	TCAACGATTCATATACACTAAGACAA	TGAGATTGGTTGACATTGGCA	30.8	42.9	56.6	58.1	1223 bp	1.0	40.7
psbA_p4	TTCAACGATTCATATACACTAAGACA	TGAGATTGGTTGACATTGGCA	30.8	42.9	56.6	58.1	1224 bp	1.0	40.7
psbA_p5	TCAACGATTCATATACACTAAGACA	GAGATTGGTTGACATTGGCA	32.0	45.0	55.9	56.3	1222 bp	1.0	40.7